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spotfire decision site microarray analysis  (TIBCO)


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    TIBCO spotfire decision site microarray analysis
    ( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
    Spotfire Decision Site Microarray Analysis, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spotfire decision site microarray analysis/product/TIBCO
    Average 90 stars, based on 1 article reviews
    spotfire decision site microarray analysis - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Role of TNF in the Altered Interaction of Dormant Mycobacterium tuberculosis with Host Macrophages"

    Article Title: Role of TNF in the Altered Interaction of Dormant Mycobacterium tuberculosis with Host Macrophages

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095220

    ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
    Figure Legend Snippet: ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

    Techniques Used: Microarray, Infection, Expressing, Isolation, Quantitative RT-PCR, Cytokine Assay, Confocal Microscopy, Derivative Assay



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    spotfire decision site microarray analysis  (TIBCO)
    90
    TIBCO spotfire decision site microarray analysis
    ( A ) . <t>Microarray</t> . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.
    Spotfire Decision Site Microarray Analysis, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spotfire decision site microarray analysis/product/TIBCO
    Average 90 stars, based on 1 article reviews
    spotfire decision site microarray analysis - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    spotfire software v.9 decision site for microarray analysis software  (TIBCO)
    90
    TIBCO spotfire software v.9 decision site for microarray analysis software
    Validation of <t>microarray</t> data . The mean ratios (expression at E11.5 up to P7/expression at E11.5) for some of the differentially expressed (DE) genes ( Amelx , Ambn , Enam , Wif1 , Clu , Prnp , Mmp20 , Col1a1 , Tgfb1 , Wint6 , and Serpinb5 ) from the microarray data (black) and real-time RT-PCR (gray). Plotted microarray and real-time RT-PCR data are means derived from at least three biological triplicates at each time point and presented with SD.
    Spotfire Software V.9 Decision Site For Microarray Analysis Software, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spotfire software v.9 decision site for microarray analysis software/product/TIBCO
    Average 90 stars, based on 1 article reviews
    spotfire software v.9 decision site for microarray analysis software - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

    Journal: PLoS ONE

    Article Title: Role of TNF in the Altered Interaction of Dormant Mycobacterium tuberculosis with Host Macrophages

    doi: 10.1371/journal.pone.0095220

    Figure Lengend Snippet: ( A ) . Microarray . Venn diagram shows the total number of genes perturbed in Mtb -H or Mtb -A infected- relative to uninfected-Rh-BMDMs. Total 226 genes (164 genes induced (UP), 62 genes repressed (DN) upon infection with Mtb -A; 2996 genes (1474 genes induced (UP), 1522 genes repressed (DN) upon infection with Mtb -H). Common genes (from up- or down-regulated gene dataset in both Mtb -H and -A group) are shown with overlap. For a description of the common genes e.g. RIPK4 , see . Heat-map clusters; green, lower expression; red, higher expression. The data are shown from independent experiments with Rh-BMDMs isolated from two Rhesus macaques. ( B ) RT-qPCR. The relative fold change in transcripts ( Mtb -H infected Rh-BMDM to Mtb -A infected Rh-BMDM) in microarray (grey bars) and RT-qPCR (white bars) is shown. The relative fold change values ( Mtb -H to Mtb -A) microarray and RT-qPCR (within bracket) are shown below; TNF, 5.3 (14.42); IL5, 3.49 (1.38); CASP8AP2, 2.93 (2.71); CXCL10, −5.7 (−8.7). ( C ) Cytokine Assay and Multilabel confocal microscopy. Measurements of TNF in supernatants, Mtb -H (red) or Mtb -A (green). Experiment was performed in triplicate and values were plotted using GraphPad Prism version 6.0b. The data is statistically significant; Student’s t-test, **P = 0.0027. Confocal microscopy shows secretion of TNF (green signal) detected only in the Rh-BMDMs (blue signal) infected with Mtb -H (red signal) (top panels) at 24 hr and 72 hr. The results are shown from Rh-BMDMs derived from two rhesus macaques.

    Article Snippet: Briefly, we used GenePix 4000B to scan chip images, GenePix Pro 6.0 to acquire raw data and Spotfire Decision Site for Microarray Analysis (TIBCO-Spotfire Inc) to perform data analysis using protocols that have been developed by us .

    Techniques: Microarray, Infection, Expressing, Isolation, Quantitative RT-PCR, Cytokine Assay, Confocal Microscopy, Derivative Assay

    Validation of microarray data . The mean ratios (expression at E11.5 up to P7/expression at E11.5) for some of the differentially expressed (DE) genes ( Amelx , Ambn , Enam , Wif1 , Clu , Prnp , Mmp20 , Col1a1 , Tgfb1 , Wint6 , and Serpinb5 ) from the microarray data (black) and real-time RT-PCR (gray). Plotted microarray and real-time RT-PCR data are means derived from at least three biological triplicates at each time point and presented with SD.

    Journal: Frontiers in Genetics

    Article Title: Gene Expression Profiling during Murine Tooth Development

    doi: 10.3389/fgene.2012.00139

    Figure Lengend Snippet: Validation of microarray data . The mean ratios (expression at E11.5 up to P7/expression at E11.5) for some of the differentially expressed (DE) genes ( Amelx , Ambn , Enam , Wif1 , Clu , Prnp , Mmp20 , Col1a1 , Tgfb1 , Wint6 , and Serpinb5 ) from the microarray data (black) and real-time RT-PCR (gray). Plotted microarray and real-time RT-PCR data are means derived from at least three biological triplicates at each time point and presented with SD.

    Article Snippet: Profile search function of Spotfire software v.9 Decision Site for Microarray analysis software (TIBCO Spotfire, Somerville, MA, USA) was used to select differentially expressed (DE) genes with a similar expression pattern to that of pre-selected genes Ambn , Amelx , and Enam .

    Techniques: Biomarker Discovery, Microarray, Expressing, Quantitative RT-PCR, Derivative Assay